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May 26, 2025

HUMAP Urine Hormone & Metabolite Test: Complete 2025 Guide

The HUMAP panel delivers a 24-hour snapshot of sex-steroid, adrenal, and detox pathways, giving clinicians and bio-hackers precise data to correct hormone imbalances, reduce cancer risk, and personalize therapy.

HUMAP Urine Hormone & Metabolite Test: Complete 2025 Guide

The HUMAP Test: A Deep Dive Into Urinary Hormone & Metabolite Profiling for Precision Wellness (2025 Guide)

1 | Why Hormone Metabolites Matter in 2025

• Hormone-responsive cancers remain a leading cause of death; urinary ratios such as 2-OHE1 : 16-OHE1 correlate with breast-cancer risk and all-cause mortality.
• Endocrine-disrupting chemicals (EDCs), chronic stress, and circadian misalignment are driving record rates of estrogen dominance, low-T, adrenal burnout, and PCOS.
• Conventional serum tests capture a fleeting “snapshot.” Urinary metabolomics reveals the whole movie—how hormones are produced, biotransformed, and eliminated over 24 hours.

Bottom line: In a data-driven era, relying on single serum estradiol or cortisol readings is like judging a Netflix series from one frame. HUMAP supplies the director’s cut.

2 | What Exactly Is the HUMAP Test?

HUMAP (Hormone & Urinary Metabolites Assessment Profile) is a multi-spot or 24-hour urine panel that quantifies 40+ parent hormones, downstream metabolites, detox ratios, and select organic acids using gold-standard liquid-chromatography tandem mass-spectrometry (LC-MS/MS).

  • Non-invasive; no phlebotomy required.

  • Captures diurnal hormone dynamics.

  • Optional neurotransmitter module adds dopamine, serotonin, GABA, and more.

Unlike single-analyte “add-ons,” HUMAP’s algorithm normalizes results to creatinine, correcting for hydration variations and enabling apples-to-apples tracking.

3 | Science Under the Hood: LC-MS/MS + Creatinine Indexing

  1. Acidified urine is divided into four aliquots representing waking, mid-morning, afternoon, and bedtime (or pooled for 24-h capture).

  2. Samples undergo solid-phase extraction to isolate free and conjugated steroids.

  3. Conjugates are enzymatically de-conjugated, providing total hormone output.

  4. LC-MS/MS quantifies analytes to picogram sensitivity, outperforming immunoassays plagued by cross-reactivity.

  5. Results are indexed per gram of creatinine, standardizing for urine concentration.

Why LC-MS/MS?
• Detects isobaric compounds (e.g., cortisol vs. cortisone).
• Delivers < 5 % coefficient of variation.
• Simultaneously measures methoxy-estrogens critical for COMT insight.

4 | Analytes at a Glance

4.1 Sex-Steroid Pathways

Parent Hormone Key Metabolites (Phase I) Downstream (Phase II) Clinical Pearl
Estrone (E1) 2-OHE1, 4-OHE1, 16-OHE1 2-MeOE1, 4-MeOE1 Low 2 : 16 ratio (< 1.5) linked to ER+ cancer risk 
Estradiol (E2) 2-OHE2, 4-OHE2 2-MeOE2, 4-MeOE2 High 4-OHE2 suggests DNA-adduct potential
Progesterone Pregnanediol, Allopregnanolone — Low luteal PdG flags infertility
Testosterone Androsterone, Etiocholanolone 11-keto metabolites Elevated 5α-reduced T may drive acne/alopecia

4.2 Adrenal Stress & Cortisol Metabolites

  • Free cortisol × 4 points + tetrahydrocortisol (THF), tetrahydrocortisone (THE) reveal both production and clearance.

  • Cortisol:Cortisone ratio illuminates 11β-HSD activity—key in insulin resistance.

4.3 Phase I vs. Phase II Detox Ratios

Ratio Interpretation
2-OHE1 : 16-OHE1 > 2.0 = protective; < 1.5 = estrogen dominance. 
2-MeOE1 : 2-OHE1 Reflects COMT methylation; low ratio suggests need for SAMe, folate, B-vits.
Androsterone : Etiocholanolone Indicates 5α- vs 5β-reductase bias impacting prostate and hair.

4.4 Methylation Efficiency (COMT)

• COMT converts reactive catechols to benign methoxy-estrogens.
• HUMAP quantifies both substrate and product, offering a functional snapshot of COMT activity—vital for patients with COMT V158M polymorphism. 

4.5 Neurotransmitter & Nutrient Add-Ons

Optional slide-outs measure:

  • Dopamine, Norepinephrine, Epinephrine

  • Serotonin, 5-HIAA

  • GABA, Glutamate

  • Kynurenic vs. Quinolinic acid (neuro-inflammation markers)

  • Vitamin B6, B12, Folate co-factor markers via organic acids

5 | Who Should Consider Ordering HUMAP?

Patient Profile Why HUMAP Helps
Peri- & post-menopausal women Tailors bio-identical HRT, monitors cancer-protective 2-OH pathway.
Men 40+ with fatigue or low libido Distinguishes primary hypogonadism from high aromatase-driven estrogen excess.
PCOS & infertility cases Maps androgen spillover and progesterone deficiency in one test.
Endometriosis, fibroids, painful periods Validates estrogen dominance and sluggish methylation.
Athletes & bio-hackers Confirms over-training cortisol patterns, guides adaptogen dosing.
Breast / Prostate cancer survivors Surveillance of estrogen or DHT metabolites without radiation exposure.
Histamine intolerance, anxiety Low COMT activity → catechol buildup; results prompt SAMe/ Mg / B-vitamine protocols.

6 | Seven High-Impact Clinical Use-Cases

  1. Personalized HRT Titration
    Example: A 55-y-o on transdermal E2 has optimal serum estradiol (60 pg/mL) yet develops fibrocystic breasts. HUMAP shows 4-OHE1 elevated and 4-MeOE1 low. Clinician adds methyl-B12/B6 and DIM; follow-up panel normalizes ratios—symptoms resolve.

  2. PCOS Root-Cause Mapping
    HUMAP reveals high androsterone and low progesterone metabolites, focusing treatment on ovulation support, myo-inositol, and spearmint extract rather than oral contraceptives.

  3. Stress-Induced Weight Gain
    Elevated midnight free cortisol + high THF/THE ratio guide phosphatidylserine + timed blue-light exposure. Weight stalls reverse.

  4. Autoimmune Thyroid + Estrogen Dominance
    Low 2:16 ratio with sluggish COMT prompts liver-support and selenium; Hashimoto antibodies drop 30 % in 6 months.

  5. Male Androgenic Alopecia
    High 5α-reductase (androsterone bias) flags dutasteride vs. finasteride decision. HUMAP follow-up confirms efficacy.

  6. Migraine with Aura
    Severe luteal estrogen drop and low SAMe ratio uncovered—methyl-folate + topical E2 patch stabilize cycle and abolish migraines.

  7. Oncology Recurrence Monitoring
    Quarterly HUMAP panels in ER+ breast-cancer survivor guide weight loss, flaxseed lignans, and calcium-D-glucarate, maintaining protective metabolic ratios for 4 years.

7 | Collection Logistics, Turn-Around, and Cost

Parameter Details
Sample type 4-spot dried urine cards or chilled 24 h liquid collection.
Preparation Avoid alcohol & supplements 48 h; freeze if shipping > 3 days.
Analytical TAT 7–10 business days portal delivery.
Retail cost $279-$389 depending on add-on labs
CPT codes 82542×N (each LC-MS analyte) or bundled 80299; check payer policy.
Insurance Not covered, you can try out-of-network self-filing

8 | Interpreting Results—From Raw Numbers to Action Plans

Step 1: Check Reference Ranges (sex- and age-specific).
Step 2: Analyze Ratios

  • 2-OH vs. 16-OH for estrogen safety.

  • 5α vs. 5β for androgen dominance.

  • Free vs. metabolized cortisol for adrenal reserve.

Step 3: Overlay Symptoms
Lab data without clinical context = noise. Match patterns: estrogen dominance symptoms + low 2 : 16 ratio validates plan.

Step 4: Craft Intervention

Imbalance Evidence-Based Fix
Low 2 : 16 ratio DIM 200 mg, crucifers, flaxseed lignans, magnesium.
Sluggish COMT Methyl-B12 1 mg, methyl-folate 1 mg, SAMe 200 mg.
High 5α reductase Saw palmetto 320 mg, nettle root, dutasteride.
Cortisol > 17 μg/g Cr overnight Phosphatidylserine 200 mg HS, ashwagandha, CBT-I.

Step 5: Re-Test in 3–6 Months to document progress, adjust doses, and improve adherence.

9 | HUMAP vs. Other Urine Hormone Tests

Feature HUMAP DUTCH Complete Basic 24-h Urine
# Metabolites 40+ 35 10-15
LC-MS/MS ✔ ✔ Some labs ELISA
COMT methylation ratios ✔ Partial ✖
Creatinine normalization ✔ ✔ Variable
Neurotransmitter add-on ✔ ✖ ✖
Report usability Heat-map + algorithmic summary Graphical charts Numeric only

Why choose HUMAP? Broader analyte panel, COMT insights, and neurotransmitter option make it a swiss-army knife for integrative practices.

10 | Limitations, Insurance, and Best-Practice Consent

  • Not a stand-alone diagnostic for cancer—should complement imaging/biopsy.

  • Phase III metabolites (glucuronidation, sulfation) not directly measured; consider genetics or serum bilirubin for deeper detox insight.

  • Some SSRI/SNRI meds skew neurotransmitter readings; note in chart.

  • FDA categorizes HUMAP as an LDT; quality relies on CLIA & CAP accreditation, not pre-market approval.

  • Obtain informed consent outlining off-label nature and potential out-of-pocket cost.

11 | Key Take-Home Points

  1. HUMAP offers the most comprehensive urinary view of hormones and metabolites available in 2025.

  2. It excels for estrogen-dominance, PCOS, HRT titration, adrenal evaluation, and cancer-risk surveillance.

  3. LC-MS/MS accuracy plus creatinine indexing ensure high reproducibility for longitudinal tracking.

  4. Results translate into actionable nutrition, lifestyle, and supplement protocols, driving personalized medicine forward.

  5. Pair HUMAP with follow-up panels every 3–6 months to verify interventions, optimize dosing, and empower patient engagement.

12 | Scientific References

  1. Doctor’s Data. Hormone & Urinary Metabolites Assessment Profile (HUMAP) Technical Guide. 2025. (Doctor's Data, Doctor's Data)

  2. Rupa Health. HuMAP™ Overview and Sample Report. 2025. (Rupa Health, Life Extension)

  3. Moore SC et al. Urinary estrogen metabolites and breast-cancer risk. J Clin Oncol. 2024. (DUTCH Test)

  4. ZRT Laboratory. Estrogen Metabolites Information Guide. 2024. (ZRT Laboratory)

  5. Bradlow HL et al. 2-Hydroxyestrone:16α-Hydroxyestrone ratio and breast-cancer risk. Endocr Relat Cancer. 2023. (PMC)

  6. Sánchez-Blanco C et al. COMT polymorphisms in hormone metabolism and breast cancer. Cancer Letters. 2025. (PMC)

  7. Fitzgerald K. Urinary hormone metabolite testing and breast-cancer prevention. Integr Med Rev. 2025. (Dr. Kara Fitzgerald)

  8. Life Extension Labs. Hormone & Urinary Metabolites Assessment Profile. 2024. (Life Extension)

  9. RN Labs. HUMAP Plus Neurotransmitters Patient Guide. 2025. (RN Labs)

  10. Baritz Wellness. Measuring Urinary Estrogen Metabolites for Cancer Risk Assessment. 2024. (Baritz Wellness)